Drug screening in vitro cancer cell lines and in vivo zebrafish
Zebrafish has become an important model for testing anticancer activity of drugs using toxicity and xenotransplantation studies. We work towards developing new methodologies for drug testing using breast and hepatocellular cancer cell lines and novel derivatives of indolebenzimidazoles.
Comparative transcriptomics for understanding of cellular signaling pathways
We perform comparative expression profiling among cell lines and gene network analyses to understand functional implications of cholinergic signaling in cancer and neuronal cells. Functional analyses we conduct include validation of microarray results at the mRNA, protein and metabolic activity as well as development of in vivo validation models using zebrafish xenotransplant and transgenic animal studies.
Development of methods for statistical, functional and meta analysis of microarray datasets
Research in the lab focuses on finding novel ways for meta-analysis of expression datasets for mRNA and microRNAs through development of web interfaces for multivariate visualization, data extraction and gene prioritization. We are also interested in comparing transcriptomes of zebrafish and mammals for deciphering conserved signaling pathways in response to anticancer drugs and other modulators of physiology.